Properties of Partially Purified .BETA.-N-Acetylglucosaminidase from Bovine Crystalline Lens.
نویسندگان
چکیده
منابع مشابه
Purification and properties of beta-N-acetylglucosaminidase from Escherichia coli.
beta-N-acetylglucosaminidase (EC 3.2.1.30) has been purified from Escherichia coli K-12 to near homogeneity based on polyacrylamide gel electrophoresis in both 0.5% sodium dodecyl sulfate and in 6 M urea at pH 8.5. The purified enzyme shows a pH optimum of 7.7 and the Km for p-nitrophenyl-beta-D-2-acetamido-2-deoxyglucopyranoside is 0.43 mM. The molecular weight of this enzyme, determined by bo...
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آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...
15 صفحه اولProperties of deoxycytidine kinase partially purified from L1210 cells.
A kinase that catalyzes the phosphorylation of deoxycytidine has been purified 150-fold from L1210 murine leukemia cells. The reaction requires a divalent cation (preferably Mg++ or Mn++) and a nucleoside triphosphate. Optimal activity was obtained with ATP. Phosphorylation of deoxycytidine was competitively inhibited by both proximal (dCMP) and distal (dCDP and dCTP) end products and by cytosi...
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The activity of deoxythymidine kinase appears related to the proliferative ability of the cell (l-6). In adult rat liver, deoxythymidine kinase activity is virtually undetectable but appears rapidly during liver regeneration (I, 5, 7-10); the enhancement in the latter case is completely blocked by X-irradiation (II). Deoxythymidine kinase activity is also markedly enhanced in the livers of rats...
متن کاملProperties of partially purified carnitine acetyltransferase.
Subsequent reports from our laboratory showed that both carnitine (/3-hydroxy-y-trimethylammonium butyrate) and acetylcarnitinei increased oxygen uptake and oxidation of long chain fatty acids by heart homogenates incubated under conditions optimal for fatty acid oxidation (2, 3). Acetylcarnitine action could not be attributed simply to formation of carnitine via hydrolysis because acetylcarnit...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 1999
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.22.866